Pinnacle Biological Sciences

(ISSN: 2360-9478)

July 2014, Vol.2 (3).

© Author(s) 2014. This work is distributed under the Creative Commons Attribution 3.0 License.

Research Article

 

Azospirillum brasilense mutant affected in motility, nitrogen fixation, flocculation and exopolysaccharide (EPS) production

Luis Javier Martínez Morales*, Lucía Soto Urzúa, María de los Ángeles Martínez Martínez & Mariana Rosas Olvera

*Centro de Investigaciones en Ciencias Microbiológicas,
Instituto de Ciencias, Benemérita,
Universidad Autónoma de Puebla,
Edif 103J, Av. San Claudio y 24 Sur. Colonia Jardines de San,
Manuel C.P. 72570. Puebla, Pue. México.

Accepted 23 June, 2014; Available Online 15 July, 2014

Abstract:

Members of genus Azospirillum are capable of nitrogen fixation under microaerophilic conditions in association with the roots of several grasses, capsular and exocellular polysaccharides (PS), are responsible for the binding of Congo red dye to Azospirillum cells, the PS are also involved in formation of cellular aggregates. Tn5gusA was used to generate an Azospirillum brasilense UAP 154/6-51 mutant, was analyzed in activity of β-glucuronidase. The cell surface of the mutant was unable to bind Congo red dye (white), and was unable to cell aggregation (flocculation), it is not capable nitrogen fixing and does not demonstrate any motility. The present paper describe these phenotype differences and demonstrates that a single mutational event is involved, that is it a pleiotrophic mutant.


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