Pinnacle Biochemistry Research

March 2014, Vol.1 (1).

© Author(s) 2014. This work is distributed under the Creative Commons Attribution 3.0 License.

Research Article

 

Optimization of Culture Conditions for the Production of Endoglucanase from Aspergillus sydowii using Corn Cobs

Maria Mushtaq1*, Dr. Fahmida Khatoon2 & Dr. Shiza Batool3

1Ph. D Scholar
PMAS Arid
AgricultureUniversity
Rawalpindi, Pakistan.
Lecturer
Biochemistry Department
Northern Boarder University
Ar Ar, University Kingdom of Saudi Arabia.

2&3Associate Professor
Department Of Microbiology
Liaqat College of Medicine and Dentistry
Pakistan.

3Lecturer
Biochemistry Department
Northern Boarder University
Ar Ar, Kingdom of Saudi Arabia.

Accepted 12 March, 2014; Available Online 20 March, 2014

Abstract:

Endoglucanase is one of the important industrial enzymes that can be produced from various microorganisms. Aspergillus sydowii is a thermophillic fungus that was utilized for the production of endoglucanase. Present study aimed to optimize different culture conditions; temperature, incubation period, pH, moisture level and inoculum size, for maximum enzyme production using agricultural wastes. Three substrates, corn cobs, sugarcane bagasse and rice straw were used in this study. Maximum endoglucanase production was observed from corn cobs, with enzyme activity of 2.01 IU/mL/min after 96 hrs of incubation at 70% moisture level (2.71 IU/mL/min), temperature 55oC (2.98 IU/mL/min) and pH 5.5 (IU/mL/min) and 3mL inoculum size (3.15 IU/mL/min). Sugarcane bagasse and rice straw found to be poor sources with enzyme activity 1.87 IU/mL/min and 1.99 IU/mL/min respectively.

Keywords: Endoglucanases, Rice Straw, Asperillus sydowii, Culture conditions.
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